Are there any requirements or best practices for in-use gas cylinders with respect to sq footage of lab space? I have a PI who wants to put 3 gas cylinders (2 compressed air, 1 CO2) in a very small (138 sq ft) space where there will be a light sheet microscope (the gases are for the microscope). I am fairly certain (but am short on details) the CO2 will be used as a freezing agent for very small amounts of tissue. Of course the cylinders will be secured, and due to room configuration would be out of the way of the single exit...but I cannot find anything in the regulations regarding a maximum storage capacity... I am going to bring in our compressed gas supplier to see if somehow we can use one compressed air cylinder to supply two different pressures (I am trying to get those details too..)

If your institution has a light sheet microscope I would love to talk with you!

I could not do my job without this listserv, so thanks everyone in advance.

Margaret
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