Practically, we need to use flexible tubing for the Schlenk line manipulations; so, we can insert the tubing to the Schlenk line ports and Schlenk flask adapter without exerting any pressure. Also, we should be able to rotate the flasks around, a thick and hard tubing will limit the movement and may exert a lot of strain on Schlenk line ports.
For maximum chemical manipulations flexible rubber tubing works very
well. We need to be only careful when manipulating highly-air sensitive compounds such as iron-sulfur cluster, otherwise rubber tubing should be fine..
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