So, if I am not mistaken 0.5 ug/ml is 0.197 mols per liter EtBr
this is an abstract from
Ethidium bromide: destruction and decontamination of solutions. (Armour Method ( Lunn, G.and E. Sansone)
Ethidium bromide in water, TBE buffer, Mops buffer, and cesium chloride solution may be completely degraded by reaction with sodium nitrite and hypophosphorous acid. Only non-mutagenic reaction mixtures were produced. Destruction was greater than 99.8% in all cases; the limit of detection was 0.5 micrograms ethidium bromide per milliliter of solution. Ethidium bromide also may be removed completely from the above solutions by using Amberlite XAD-16 resin. The limit of detection was 0.05 micrograms ethidium bromide per milliliter of solution (0.27 micrograms/ml when cesium chloride solution was used).
I haven't tested this next method but maybe follow this reaction at slight molar excess of the neutralizing reagents.
Lunn & Sansone Method: For each 100 ml of aqueous EtBr solution:
• Add 5% hypophosphorous acid.• Add 12 ml of 0.5 M sodium nitrite.• Stir briefly and let stand for 20 hours.• Adjust pH to 7-9 using sodium hydroxide.• Pour down drain with copious amounts of water.
-Hope this helps
John Darius Soltes CHMM
https://www.researchgate.net/post/What_is_the_best_way_to_ dispose_of_ethidium_bromide_ solutions_and_gels
www.researchgate.netWhat is the best way to dispose of ethidium bromide solutions and gels?
1. Anal Biochem. 1987 May 1;162(2):453-8. Ethidium bromide: destruction and decontamination of solutions. Lunn G, Sansone EB. Ethidium bromide in water, TBE buffer, Mops buffer, and cesium chloride solution
From: ACS Division of Chemical Health and Safety <DCHAS-L**At_Symbol_Here**PRINCETON.EDU> on behalf of Penny Manisco <pmanisco**At_Symbol_Here**G.HMC.EDU>
Sent: Thursday, August 9, 2018 5:37 PM
To: DCHAS-L**At_Symbol_Here**PRINCETON.EDU
Subject: [DCHAS-L] Dilute Ethidium Bromide Disposal in California--- For more information about the DCHAS-L e-mail list, contact the Divisional membership chair at membership**At_Symbol_Here**dchas.org Follow us on Twitter **At_Symbol_Here**acsdchasHello All,
There seems to be varying opinion on how to dispose of very dilute solutions of ethidium bromide. The concentration in question is 0.5 micrograms/ml of buffer. By hazardous waste definition, this would not be considered a hazardous waste, but I believe some of our California universities and colleges recommend filtration of the ethidium bromide before disposal at any concentration.
Any guidance would be very much appreciated.
Sincerely,
--
Penny Manisco,
Chemical Hygiene Officer
Harvey Mudd College
(909)6074217
--- For more information about the DCHAS-L e-mail list, contact the Divisional membership chair at membership**At_Symbol_Here**dchas.org Follow us on Twitter **At_Symbol_Here**acsdchas
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