If the storage tank can be sealed (or vented through a sterilizing filter) then the fluid could be circulated through a 0.2 =B5m cartridge filter between the shows. This should remove bacteria and anything larger. If viruses are the concern there are cartridge filters finer than 0.2 =B5m.

There will be some cost of procuring and setting up the system, non-trivial amount of energy to run the pump around the clock, more energy to potentially cool the liquid (the pump will heat it up), and the management of spills. I would need to check compatibility of all components with plastics like PVC and/or PP, but I think a recycled DI water system could be adapted for this experiment. The DI water systems have tanks, filtration units, pumps, and controls to run them. The cartridge would have to be replaced with the 0.2 =B5m or finer, but this should not be a huge issue.

The pre-requisite for this to work is that all components of “blood” are in solution. I would watch the amount of water added to the original “blood”.

The wading tank could be manually cleaned and disinfected between the shows. Disinfection in the absence of the “blood” should not raise any tox issues even with bleach.

Slawomir Janicki

Rather than heat, I'd look at cooling between shows.