From: "Ferm, Barret" <fermbarreta**At_Symbol_Here**SAU.EDU>
Subject: Re: [DCHAS-L] Gram Staining information
Date: April 7, 2012 9:33:18 PM EDT
Reply-To: DCHAS-L <DCHAS-L**At_Symbol_Here**MED.CORNELL.EDU>
Message-ID: <9BD19E74-2E3D-42EC-ADE3-7C8049E5A37B**At_Symbol_Here**MY.APSU.EDU>


Thank you for asking your question, Blanca. I, too, am interested in hearing replies to it. For these types of solutions I have been advised to add bleach to decolorize, and adjust pH to (5 - 8) if necessary, then down the drain. Is this okay??

Barry Ferm

On Sat, Apr 7, 2012 at 1:00 PM, Martinez, Blanca A. <BMARTINEZ4**At_Symbol_Here**my.apsu.edu> wrote:
Hello,
As a student finishing my clinical rotation as a medical laboratory scientist, I have received conflicting information regarding the proper disposal of gram stain - Crystal Violet, Gram's Iodine (mordant), Alcohol/Acetone (gen. isopropanol decolourize), and safranin. Some say proper disposal when staining human specimens is sufficient with water flushing down the drain versus collection into a carboy. Since I am still a student in the field, I do not question what is told to me however, I would like to know proper daily disposal. When looking at various MSDS for these chemicals it usually states to follow federal/state/local regulations for empty bottles/excess remainder however, daily disposal seems awfully vague. Can anyone please shed some light on the matter.

Thank you in advance,
Blanca Martinez


Sent from my iPhone



--
Invest in war, and you get war. Invest in peace, and you will get peace.

Previous post   |  Top of Page   |   Next post



The content of this page reflects the personal opinion(s) of the author(s) only, not the American Chemical Society, ILPI, Safety Emporium, or any other party. Use of any information on this page is at the reader's own risk. Unauthorized reproduction of these materials is prohibited. Send questions/comments about the archive to secretary@dchas.org.
The maintenance and hosting of the DCHAS-L archive is provided through the generous support of Safety Emporium.