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I've used Sterikon=AE plus Bioindicator, so long as you have access to a 60=B0C water bath. Simple, works well and, as Edward has pointed out below, uses Bacillus stearothermophilus spores so it's 'gold standard'. http://85.238.144.18/analytics/Micro_Manual/TEDISdata/prods/1_10274_0001_ 0002.html gives you more info, but need to Google for your local supplier. Cheers, Paul ____________________________________________ Paul Dover Resources Manager Medicinal Chemistry and Drug Action Monash Institute of Pharmaceutical Sciences Monash University (Parkville Campus) 381 Royal Parade, Parkville Victoria, 3052. Australia Phone: +61 3 9903 9551 Fax: +61 3 9903 9143 Email: Paul.Dover**At_Symbol_Here**pharm.monash.edu.au Web: http://www.pharm.monash.edu.au -----Original Message----- From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**list.uvm.edu] On Behalf Of McGrath Edward J Sent: Friday, 9 July 2010 5:17 AM To: Paul Dover Subject: Re: [DCHAS-L] Biological Indicators The gold standard for this protocol is Bacillus stearothermophilus endospores. This is a bacterium that only grows at temperatures above 56 degrees C (can't seem to do symbols!) Autoclaving at 121 degrees C for 15 minutes is sufficient to kill the spores. After autoclaving, you inoculate appropriate medium and incubate in a hot water bath, with a control culture of un-autoclaved spores (which should, of course, grow). I'm afraid I have no idea of pricing. Another option is autoclave tape, which reveals writing after autoclaving. You should use this with every autoclave run (it usually says "autoclaved" or "sterile.") This is good for showing which medium has been sterilized and which hasn't (assuming the medium is not compromised.) The disadvantage to the tape is that with age, the writing can appear without autoclaving. Edward J. McGrath Science Supervisor Red Clay Consolidated School District office: (302) 552-3768 -----Original Message----- From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**list.uvm.edu] On Behalf Of Beth Welmaker Sent: Thursday, July 08, 2010 2:00 PM To: DCHAS-L**At_Symbol_Here**LIST.UVM.EDU Subject: [DCHAS-L] Biological Indicators I am looking for a recommendation for simple Biological Indicators to verify our autoclave sterilization effectiveness. There are several vial styles to choose from. I don't want to waste money on a poor product. Thank you in advance! Beth Welmaker, CCHO Safety Officer 11350 SW Village Parkway - Third Floor Port Saint Lucie, FL=A0 34987 772-345-4826
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